CBCRI
Clout Bio for Research & Innovation
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Course Details

Industry-ready biotechnology training.

Structured 1-month and 2-month hands-on tracks designed to move learners from core laboratory techniques toward independent execution, data interpretation and industry-style reporting.

Module 01 • Animal Cell Culture

Preclinical Validation Assays in Animal Cell Culture

Hands-on training in mammalian cell culture, functional cell-based assays, and cellular differentiation, with relevance to application and preclinical validation.

1-Month Program

  • Laboratory induction & biosafety
  • Mammalian cell culture & aseptic techniques
  • Culture media preparation
  • Cell revival & maintenance
  • Cell counting & subculturing
  • Cryopreservation & cell banking
  • MTT cell viability assay
  • Cell migration / wound-healing assay
  • Nucleic acid isolation
  • cDNA synthesis (END POINT PCR)
  • PCR and visualization
  • Data analysis, interpretation & industry-style reporting

2-Month Program

  • All 1-month techniques
  • THP-1 cell differentiation
  • Adipogenesis assay
  • Oil Red O staining & analysis
  • Cell-based anti-inflammatory assay with imaging
  • Antioxidant assays
  • Anti-inflammatory protein expression analysis
  • Protein isolation and estimation
  • SDS-PAGE & Western blot
  • Data analysis, interpretation & industry-style reporting
Module 02 • Molecular Biology

Genetic Engineering for Genome Biologists

Hands-on training in molecular biology and genetic engineering for genome biologists, laying the foundation for biomanufacturing and synthetic biology approaches.

1-Month Program

  • Molecular biology laboratory practices & GLP
  • DNA isolation & QA/QC
  • PCR
  • Agarose gel electrophoresis
  • CaCl₂-mediated bacterial transformation
  • Antibiotic selection
  • Blue-white screening
  • Recombinant colony selection
  • Plasmid isolation
  • Restriction enzymes and digestion
  • Data analysis & reporting

2-Month Program

  • All 1-month techniques
  • Mammalian cell transfection
  • Expression plasmid handling
  • Transfection efficiency
  • Selection of transfected cells
  • SDS-PAGE
  • Western blotting
  • Target protein expression analysis
  • Protein quantification
  • Data analysis, interpretation & industry-style reporting
Module 03 • Microbiology

Microbes for Industry & Its Validation

Hands-on training in industrial microbiology, microbial isolation, characterization, culture maintenance, functional screening, and microbial quality assessment.

1-Month Program

  • Microbiology laboratory practices
  • GLP & aseptic techniques
  • Microbial isolation & pure culture preparation
  • Culture maintenance
  • Staining techniques & microscopic visualization
  • Basic biochemical characterization
  • 16S rRNA gene amplification & microbial identification
  • Antibacterial screening: agar well & disc diffusion
  • MIC assay
  • Acid & bile tolerance assay
  • Biofilm inhibition assay
  • Data analysis, interpretation & industry-style reporting

2-Month Program

  • All 1-month techniques
  • Industrial enzyme production & characterization
  • Amylase-producing strain screening
  • Amylase production
  • Enzyme extraction, recovery & concentration
  • Dialysis
  • Enzyme purification
  • Protein estimation
  • Amylase activity assay
  • Enzyme quantification & specific activity
  • Enzyme kinetics – Km and Vmax
  • Enzyme yield & recovery analysis
  • Industry-style enzyme production report

Choose your training pathway.

Register your interest and CBCRI will contact you regarding the program.
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